An aerotaxis transducer gene from Pseudomonas putida

FEMS Microbiol Lett. 2000 Jan 1;182(1):177-83. doi: 10.1111/j.1574-6968.2000.tb08893.x.

Abstract

An aerotaxis gene, aer, was cloned from Pseudomonas putida PRS2000. A P. putida aer mutant displayed an altered aerotactic response in a capillary assay. Wild-type P. putida clustered at the air/liquid interface. In contrast, the aer mutant did not cluster at the interface, but instead formed a diffuse band at a distance from the meniscus. Wild-type aer, provided in trans, complemented the aer mutant to an aerotactic response that was stronger than wild-type. The P. putida Aer sequence is similar over its entire length to the aerotaxis (energy taxis) signal transducer protein, Aer, of Escherichia coli. The amino-terminus is similar to redox-sensing regulatory proteins, and the carboxy-terminus contains the highly conserved domain present in chemotactic transducers.

Publication types

  • Research Support, U.S. Gov't, P.H.S.

MeSH terms

  • Carrier Proteins / chemistry
  • Carrier Proteins / genetics*
  • Chemotaxis / genetics
  • Cloning, Molecular
  • Culture Media
  • Energy Metabolism / physiology
  • Escherichia coli Proteins*
  • Genes, Bacterial*
  • Intercellular Signaling Peptides and Proteins
  • Kanamycin Resistance / genetics
  • Molecular Sequence Data
  • Mutagenesis, Insertional
  • Pseudomonas putida / genetics*
  • Pseudomonas putida / physiology*
  • Signal Transduction / genetics
  • Succinic Acid / metabolism

Substances

  • Aer protein, E coli
  • Carrier Proteins
  • Culture Media
  • Escherichia coli Proteins
  • Intercellular Signaling Peptides and Proteins
  • Succinic Acid

Associated data

  • GENBANK/AF079997