Reduced flux through the purine biosynthetic pathway results in an increased requirement for coenzyme A in thiamine synthesis in Salmonella enterica serovar typhimurium

J Bacteriol. 2000 Jan;182(1):236-40. doi: 10.1128/JB.182.1.236-240.2000.


Work presented here establishes a connection between cellular coenzyme A (CoA) levels and thiamine biosynthesis in Salmonella enterica serovar Typhimurium. Prior work showed that panE mutants (panE encodes ketopantoate reductase) had a conditional requirement for thiamine or pantothenate. Data presented herein show that the nutritional requirement of panE mutants for either thiamine or pantothenate is manifest only when flux through the purine biosynthetic pathway is reduced. Further, the data show that under the above conditions it is the lack of thiamine pyrophosphate, and not decreased CoA levels, that directly prevents growth.

Publication types

  • Research Support, U.S. Gov't, P.H.S.

MeSH terms

  • Adenine / metabolism
  • Alcohol Oxidoreductases / genetics
  • Alcohol Oxidoreductases / metabolism
  • Cell Division / genetics
  • Coenzyme A / metabolism*
  • Culture Media
  • Mutation
  • Pantothenic Acid / metabolism
  • Purines / biosynthesis*
  • Salmonella typhimurium / genetics
  • Salmonella typhimurium / growth & development
  • Salmonella typhimurium / metabolism*
  • Thiamine / biosynthesis*
  • Thiamine Pyrophosphate / metabolism


  • Culture Media
  • Purines
  • Pantothenic Acid
  • Alcohol Oxidoreductases
  • 2-dehydropantoate 2-reductase
  • Adenine
  • Thiamine Pyrophosphate
  • Coenzyme A
  • Thiamine