Sequence and Analysis of Chromosome 2 of the Plant Arabidopsis Thaliana

Nature. 1999 Dec 16;402(6763):761-8. doi: 10.1038/45471.

Abstract

Arabidopsis thaliana (Arabidopsis) is unique among plant model organisms in having a small genome (130-140 Mb), excellent physical and genetic maps, and little repetitive DNA. Here we report the sequence of chromosome 2 from the Columbia ecotype in two gap-free assemblies (contigs) of 3.6 and 16 megabases (Mb). The latter represents the longest published stretch of uninterrupted DNA sequence assembled from any organism to date. Chromosome 2 represents 15% of the genome and encodes 4,037 genes, 49% of which have no predicted function. Roughly 250 tandem gene duplications were found in addition to large-scale duplications of about 0.5 and 4.5 Mb between chromosomes 2 and 1 and between chromosomes 2 and 4, respectively. Sequencing of nearly 2 Mb within the genetically defined centromere revealed a low density of recognizable genes, and a high density and diverse range of vestigial and presumably inactive mobile elements. More unexpected is what appears to be a recent insertion of a continuous stretch of 75% of the mitochondrial genome into chromosome 2.

Publication types

  • Research Support, U.S. Gov't, Non-P.H.S.

MeSH terms

  • Arabidopsis / genetics*
  • Cell Nucleus / genetics
  • Centromere
  • Chromosome Mapping*
  • DNA, Plant*
  • Evolution, Molecular
  • Gene Duplication
  • Genes, Plant* / physiology
  • Mitochondria / genetics
  • Molecular Sequence Data
  • Plant Proteins / genetics
  • Plant Proteins / physiology
  • Sequence Analysis, DNA

Substances

  • DNA, Plant
  • Plant Proteins

Associated data

  • GENBANK/AC002329
  • GENBANK/AC002332
  • GENBANK/AC002333
  • GENBANK/AC002334
  • GENBANK/AC002335
  • GENBANK/AC002336
  • GENBANK/AC002337
  • GENBANK/AC002338
  • GENBANK/AC002339
  • GENBANK/AC002340
  • GENBANK/AC002341
  • GENBANK/AC002354
  • GENBANK/AC002387
  • GENBANK/AC002388
  • GENBANK/AC002391
  • GENBANK/AC002392
  • GENBANK/AC002409
  • GENBANK/AC002505
  • GENBANK/AC002510
  • GENBANK/AC002521
  • GENBANK/AC002535
  • GENBANK/AC002561
  • GENBANK/AC003000
  • GENBANK/AC003028
  • GENBANK/AC003033
  • GENBANK/AC003040
  • GENBANK/AC003058
  • GENBANK/AC003096
  • GENBANK/AC003105
  • GENBANK/AC003672