Development of positive selectable markers for the fungal pathogen Cryptococcus neoformans

Clin Diagn Lab Immunol. 2000 Jan;7(1):125-8. doi: 10.1128/CDLI.7.1.125-128.2000.


Cryptococcus neoformans is an opportunistic fungal pathogen that causes meningitis in approximately 10% of patients with AIDS. New selectable markers which confer resistance to G418 or phleomycin when transformed into C. neoformans were made. A hygromycin-selectable marker was modified to allow selection with a single copy of the marker.

Publication types

  • Research Support, Non-U.S. Gov't
  • Research Support, U.S. Gov't, P.H.S.

MeSH terms

  • Anti-Bacterial Agents / pharmacology*
  • Biomarkers / chemistry*
  • Cryptococcus neoformans / immunology*
  • Cryptococcus neoformans / isolation & purification*
  • Drug Resistance, Microbial / physiology
  • Gentamicins / pharmacology
  • Phleomycins / pharmacology
  • Serotyping


  • Anti-Bacterial Agents
  • Biomarkers
  • Gentamicins
  • Phleomycins
  • antibiotic G 418