Evaluation of neutrophil structure and function by electron microscopy: cytochemical studies

J M Robinson; T Kobayashi; H Seguchi; T Takizawa

doi:10.1016/s0022-1759(99)00174-x

Evaluation of neutrophil structure and function by electron microscopy: cytochemical studies

J Immunol Methods. 1999 Dec 17;232(1-2):169-78. doi: 10.1016/s0022-1759(99)00174-x.

Authors

J M Robinson¹, T Kobayashi, H Seguchi, T Takizawa

Affiliation

¹ Department of Physiology and Cell Biology, Ohio State University, 302 Hamilton Hall, 1645 Neil Ave., Columbus, OH 43210, USA. robinson.21@osu.edu

PMID: 10618518
DOI: 10.1016/s0022-1759(99)00174-x

Abstract

Methods for studying human neutrophils at the ultrastructural level by enzyme cytochemistry and immunocytochemistry are presented. The focus of these methods is on the analysis of the alkaline phosphatase-positive secretory organelle of these cells. These methods provide unique information which, when coupled with biochemical studies, provide for the most complete analysis of neutrophil structure and function.

MeSH terms

Alkaline Phosphatase / metabolism
Cytoplasmic Granules / enzymology
Cytoplasmic Granules / immunology
Cytoplasmic Granules / ultrastructure
Humans
Immunohistochemistry
Interphase / immunology
Microscopy, Electron / methods*
Microscopy, Immunoelectron
Neutrophil Activation / immunology
Neutrophils / chemistry*
Neutrophils / enzymology
Neutrophils / immunology
Neutrophils / ultrastructure*
Oxygen / metabolism
Superoxides / metabolism

Substances

Superoxides
Alkaline Phosphatase
Oxygen