A C/EBP Beta Isoform Recruits the SWI/SNF Complex to Activate Myeloid Genes

Mol Cell. 1999 Nov;4(5):735-43. doi: 10.1016/s1097-2765(00)80384-6.

Abstract

The activation of many genes requires the concerted effort of two or more transcription factors. Although C/EBP beta is known to cooperate with Myb to induce transcription of the granulocyte-specific mim-1 gene, the molecular mechanism of this cooperativity is undefined. We show that the N terminus of the full-length C/EBP beta isoform, which is essential for induction of the mim-1 gene in chromatin, interacts specifically with the SWI/SNF complex. Grafting this domain onto Myb generates a chimeric activator that recruits SWI/SNF and induces mim-1 transcription in the absence of C/EBP beta. Interaction between C/EBP beta and SWI/SNF is essential for activating a subgroup of resident target genes in chromatin and may represent a major determinant of combinatorial gene regulation in eukaryotes.

Publication types

  • Research Support, Non-U.S. Gov't

MeSH terms

  • Acetyltransferases*
  • Adenosine Triphosphatases
  • Animals
  • Binding Sites
  • CCAAT-Enhancer-Binding Proteins
  • Cell Cycle Proteins*
  • Cell Line
  • Chromatin / genetics
  • Chromatin / metabolism
  • DNA Helicases
  • DNA-Binding Proteins / chemistry
  • DNA-Binding Proteins / genetics
  • DNA-Binding Proteins / metabolism*
  • Drosophila Proteins
  • Erythroblasts / cytology
  • Erythroblasts / metabolism
  • Fibroblasts / cytology
  • Fibroblasts / metabolism
  • Humans
  • Multienzyme Complexes / metabolism*
  • Nuclear Proteins / chemistry
  • Nuclear Proteins / genetics
  • Nuclear Proteins / metabolism*
  • Promoter Regions, Genetic / genetics
  • Protein Binding
  • Protein Isoforms / chemistry
  • Protein Isoforms / metabolism
  • Proteins / genetics*
  • Proto-Oncogene Proteins c-myb / genetics
  • Proto-Oncogene Proteins c-myb / metabolism
  • Recombinant Fusion Proteins / chemistry
  • Recombinant Fusion Proteins / metabolism
  • Saccharomyces cerevisiae / genetics
  • Saccharomyces cerevisiae Proteins
  • Sequence Deletion / genetics
  • Trans-Activators / genetics
  • Trans-Activators / metabolism
  • Transcription Factors / genetics
  • Transcription Factors / metabolism*
  • Transcriptional Activation / genetics*

Substances

  • CCAAT-Enhancer-Binding Proteins
  • Cell Cycle Proteins
  • Chromatin
  • DNA-Binding Proteins
  • Drosophila Proteins
  • Multienzyme Complexes
  • Nuclear Proteins
  • Protein Isoforms
  • Proteins
  • Proto-Oncogene Proteins c-myb
  • Recombinant Fusion Proteins
  • SMARCA1 protein, human
  • SMARCA2 protein, human
  • Saccharomyces cerevisiae Proteins
  • Trans-Activators
  • Transcription Factors
  • brm protein, Drosophila
  • Acetyltransferases
  • mim-1 protein, Gallus gallus
  • Adenosine Triphosphatases
  • SMARCA4 protein, human
  • SNF2 protein, S cerevisiae
  • DNA Helicases