A role for presenilin-1 in nuclear accumulation of Ire1 fragments and induction of the mammalian unfolded protein response

Cell. 1999 Dec 23;99(7):691-702. doi: 10.1016/s0092-8674(00)81667-0.


The unfolded protein response (UPR) mediates signaling from the endoplasmic reticulum to the nucleus. In yeast, a key regulatory step in the UPR is the spliceosome-independent splicing of HAC1 mRNA encoding a UPR-specific transcription factor, which is initiated by the transmembrane kinase/endoribonuclease Ire1. We show that yeast HAC1 mRNA is correctly spliced in mammalian cells upon UPR induction and that mammalian Ire1 can precisely cleave both splice junctions. Surprisingly, UPR induction leads to proteolytic cleavage of Ire1, releasing fragments containing the kinase and nuclease domains that accumulate in the nucleus. Nuclear localization and UPR induction are reduced in presenilin-1 knockout cells. These results suggest that the salient features of the UPR are conserved among eukaryotic cells and that presenilin-1 controls Ire1 proteolysis in mammalian cells.

Publication types

  • Research Support, Non-U.S. Gov't
  • Research Support, U.S. Gov't, P.H.S.

MeSH terms

  • Animals
  • Basic-Leucine Zipper Transcription Factors
  • COS Cells
  • Cell Nucleus / metabolism
  • Endoplasmic Reticulum / metabolism
  • Endoplasmic Reticulum / ultrastructure
  • Endoribonucleases / metabolism*
  • Fungal Proteins / metabolism*
  • HeLa Cells
  • Humans
  • Intracellular Membranes / metabolism
  • Introns
  • Membrane Glycoproteins / metabolism*
  • Membrane Proteins / deficiency
  • Membrane Proteins / metabolism*
  • Peptide Fragments / metabolism
  • Presenilin-1
  • Protein Folding*
  • Protein Kinases / metabolism*
  • Protein-Serine-Threonine Kinases*
  • RNA Splicing
  • RNA, Messenger / metabolism
  • Repressor Proteins / metabolism*
  • Saccharomyces cerevisiae Proteins*
  • Transcription Factors*


  • Basic-Leucine Zipper Transcription Factors
  • Fungal Proteins
  • HAC1 protein, S cerevisiae
  • Membrane Glycoproteins
  • Membrane Proteins
  • PSEN1 protein, human
  • Peptide Fragments
  • Presenilin-1
  • RNA, Messenger
  • Repressor Proteins
  • Saccharomyces cerevisiae Proteins
  • Transcription Factors
  • Protein Kinases
  • IRE1 protein, S cerevisiae
  • Protein-Serine-Threonine Kinases
  • Endoribonucleases