E-Cadherin and CD44 expression in cervical intraepithelial neoplasia: comparison between HIV-positive and HIV-negative women and correlation with HPV status

Gynecol Oncol. 2000 Jan;76(1):56-62. doi: 10.1006/gyno.1999.5656.

Abstract

Aims: We aimed to compare the expression patterns of E-cadherin and CD44 isoforms in cervical intraepithelial neoplasia (CIN) between patients with or without infection by the human immunodeficiency virus (HIV).

Methods: An immunohistochemical analysis using the monoclonal antibody HECD-1 against E-cadherin and the monoclonal antibodies 2C5, binding to CD44s and all the variants encoded by exons 3 to 10, 3G5, specific for CD44v3 and 2F10, and specific for CD44v6, was performed in formalin-fixed, paraffin-embedded samples of 138 CIN (74 from HIV-negative and 64 from HIV-positive patients).

Results: In HIV-negative patients, the mean percentages (+/-SD) of E-cadherin-positive cells in CIN of grades I, II, and III were, respectively, 33% +/- 4, 63% +/- 5, and 91% +/- 9. The difference was statistically significant between the three groups of tumors (P < 0.0001). In HIV-negative patients, the mean percentages (+/-SD) of CD44-positive cells in CIN of grades I, II, and III were, respectively, 37% +/- 7, 57% +/- 8, and 90% +/- 11. The difference was statistically significant between the three groups of tumors (P < 0.0001). No difference in E-cadherin and CD44 expressions was noted between HIV+ and HIV- women. Further analysis showed no relation between E-cadherin or CD44 expression and the HPV status and CD4 T cell serum levels.

Conclusion: Our study confirms that alterations in E-cadherin and CD44 expression in CIN depend on the histological grade but suggest nondirect involvement and are not related to HIV and immune status.

MeSH terms

  • Adult
  • Biomarkers, Tumor / analysis
  • Cadherins / analysis
  • Cadherins / biosynthesis*
  • Carcinoma in Situ / immunology*
  • Carcinoma in Situ / virology*
  • Female
  • HIV Infections / immunology*
  • Humans
  • Hyaluronan Receptors / analysis
  • Hyaluronan Receptors / biosynthesis*
  • Immunohistochemistry
  • Immunosuppression
  • Middle Aged
  • Neoplasm Staging
  • Papillomaviridae / immunology*
  • Papillomavirus Infections / immunology*
  • Tumor Virus Infections / immunology*
  • Uterine Cervical Neoplasms / immunology*
  • Uterine Cervical Neoplasms / virology*

Substances

  • Biomarkers, Tumor
  • Cadherins
  • Hyaluronan Receptors