Cloning and characterization of a novel cis-naphthalene dihydrodiol dehydrogenase gene (narB) from Rhodococcus sp. NCIMB12038

FEMS Microbiol Lett. 2000 Jan 15;182(2):327-31. doi: 10.1111/j.1574-6968.2000.tb08916.x.

Abstract

Rhodococcus sp. NCIMB112038 can utilize naphthalene as its sole carbon and energy source. The gene encoding cis-naphthalene dihydrodiol dehydrogenase (narB) of this strain has been cloned and sequenced. Expression of NCIMB12038 cis-naphthalene dihydrodiol dehydrogenase was demonstrated in Escherichia coli cells. narB encodes a putative protein of 271 amino acids and shares 39% amino acid identity with the cis-naphthalene dihydrodiol dehydrogenase from Pseudomonas putida G7. Comparison of NarB with some putative cis-dihydrodiol dehydrogenases from Rhodococcus species revealed significant differences between these proteins. NarB together with two other proteins forms a new group of cis-dihydrodiol dehydrogenases.

MeSH terms

  • Alcohol Oxidoreductases / chemistry
  • Alcohol Oxidoreductases / genetics*
  • Alcohol Oxidoreductases / metabolism*
  • Amino Acid Sequence
  • Biodegradation, Environmental
  • Cell Fractionation
  • Cloning, Molecular
  • Culture Media
  • Genes, Bacterial
  • Molecular Sequence Data
  • Naphthalenes / metabolism*
  • Oxidoreductases Acting on CH-CH Group Donors*
  • Oxidoreductases*
  • Phylogeny
  • Rhodococcus / enzymology*
  • Rhodococcus / genetics*
  • Rhodococcus / growth & development
  • Sequence Alignment
  • Sequence Analysis, DNA

Substances

  • Culture Media
  • Naphthalenes
  • naphthalene
  • Oxidoreductases
  • Alcohol Oxidoreductases
  • dihydrodiol dehydrogenases
  • Oxidoreductases Acting on CH-CH Group Donors
  • cis-1,2-dihydro-1,2-dihydroxynaphthalene dehydrogenase

Associated data

  • GENBANK/AF082663