Forskolin induces circadian gene expression of rPer1, rPer2 and dbp in mammalian rat-1 fibroblasts

FEBS Lett. 2000 Jan 7;465(1):79-82. doi: 10.1016/s0014-5793(99)01724-x.


Mammalian culture cells have the potential for periodicity, since high concentrations of serum can elicit the circadian expression of clock genes in rat-1 fibroblasts. However, the mechanism by which serum affects circadian gene expression remains unclear. In the present study, we incubated rat-1 cells with forskolin and successfully induced the rhythmic expression of Per1, Per2 and dbp. In the initial step of the circadian gene expression, a marked transient induction of Per1 was observed accompanied with CREB phosphorylation. Thus the present study strongly suggests that CREB activation through the cAMP/PKA pathway is involved in the generation of circadian rhythm in rat-1 cells

Publication types

  • Research Support, Non-U.S. Gov't

MeSH terms

  • Activating Transcription Factor 2
  • Animals
  • Carrier Proteins / biosynthesis
  • Carrier Proteins / genetics*
  • Cell Cycle Proteins
  • Cells, Cultured
  • Circadian Rhythm / genetics
  • Colforsin / pharmacology*
  • Cyclic AMP Response Element-Binding Protein / metabolism
  • Dicarboxylic Acid Transporters
  • Gene Expression
  • Nuclear Proteins / biosynthesis
  • Nuclear Proteins / genetics*
  • Period Circadian Proteins
  • Phosphorylation
  • Rats
  • Transcription Factors / metabolism


  • Activating Transcription Factor 2
  • Carrier Proteins
  • Cell Cycle Proteins
  • Cyclic AMP Response Element-Binding Protein
  • Dicarboxylic Acid Transporters
  • Nuclear Proteins
  • Per1 protein, mouse
  • Per1 protein, rat
  • Per2 protein, mouse
  • Per2 protein, rat
  • Period Circadian Proteins
  • Transcription Factors
  • Colforsin