GPR1 Regulates Filamentous Growth Through FLO11 in Yeast Saccharomyces Cerevisiae

Biochem Biophys Res Commun. 2000 Jan 7;267(1):164-8. doi: 10.1006/bbrc.1999.1914.

Abstract

Cell growth and differentiation are regulated by nutrient availability in the yeast Saccharomyces cerevisiae. Under conditions of nitrogen limitation, diploid cells of S. cerevisiae differentiate to a filamentous growth known as a pseudohyphal growth, while haploid cells produce invasive filaments which penetrate the agar in nutrient-rich medium. We have found that GPR1, which encodes a putative G-protein-coupled receptor, is required for both pseudohyphal and invasive growth. Pseudohyphal growth was defective in Deltagpr1/Deltagpr1 mutant strain and this defect was reversed by addition of cAMP. Also, haploid Deltagpr1 mutant strain was defective in invasive growth. Northern blot analysis revealed that the transcriptional level of FLO11, which encodes a recently identified cell surface flocculin required for pseudohyphal growth, was reduced in Deltagpr1 mutant strain. These results indicate that GPR1 regulates both pseudohyphal and invasive growth by a cAMP-dependent mechanism.

Publication types

  • Research Support, Non-U.S. Gov't

MeSH terms

  • Blotting, Northern
  • Culture Media
  • Fungal Proteins / genetics
  • Fungal Proteins / physiology
  • GTP-Binding Protein alpha Subunits*
  • Genotype
  • Heterotrimeric GTP-Binding Proteins / genetics
  • Heterotrimeric GTP-Binding Proteins / physiology
  • Homozygote
  • Membrane Glycoproteins
  • Membrane Proteins / genetics
  • Membrane Proteins / physiology*
  • Mutagenesis, Insertional
  • Receptors, Cell Surface / genetics
  • Receptors, Cell Surface / physiology*
  • Receptors, G-Protein-Coupled*
  • Saccharomyces cerevisiae / genetics
  • Saccharomyces cerevisiae / growth & development
  • Saccharomyces cerevisiae / physiology*
  • Saccharomyces cerevisiae Proteins*

Substances

  • Culture Media
  • FLO11 protein, S cerevisiae
  • Fungal Proteins
  • GPR1 protein, S cerevisiae
  • GTP-Binding Protein alpha Subunits
  • Membrane Glycoproteins
  • Membrane Proteins
  • Receptors, Cell Surface
  • Receptors, G-Protein-Coupled
  • Saccharomyces cerevisiae Proteins
  • Gpa2 protein, S cerevisiae
  • Heterotrimeric GTP-Binding Proteins