BWMK1, a novel MAP kinase induced by fungal infection and mechanical wounding in rice

Mol Plant Microbe Interact. 1999 Dec;12(12):1064-73. doi: 10.1094/MPMI.1999.12.12.1064.

Abstract

The activation of the mitogen-activated protein (MAP) kinases by different environmental stresses has been previously observed in several dicot plant species. Here, we report the isolation of a novel MAP kinase in rice that is induced during infection by the blast fungus Magnaporthe grisea or upon mechanical wounding. The gene is designated as BWMK1 for blast- and wound-induced MAP kinase. The cDNA of BWMK1 was isolated from rice leaves challenged by the blast pathogen. Transcripts of the corresponding gene accumulated in rice leaves 4 h after blast inoculation and 30 min after mechanical wounding. This gene encodes a 506 amino acid protein that contains a new dual-phosphorylation activation motif TDY and about 150 unique amino acids on its C terminus. In-gel kinase activity and immunoprecipitation assays confirmed that BWMK1 is a functional MAP kinase. These results show that BWMK1 is a new member of the plant MAP kinase family and may mediate both defense and wound signaling in rice.

MeSH terms

  • Amino Acid Sequence
  • Antibodies, Monoclonal / immunology
  • Base Sequence
  • Cloning, Molecular
  • DNA, Complementary
  • Genome, Plant
  • Magnaporthe / physiology*
  • Mitogen-Activated Protein Kinases / biosynthesis*
  • Mitogen-Activated Protein Kinases / genetics
  • Mitogen-Activated Protein Kinases / immunology
  • Molecular Sequence Data
  • Oryza / enzymology*
  • Oryza / genetics
  • Oryza / microbiology
  • Phosphorylation
  • Phosphotyrosine / immunology
  • Plant Proteins
  • Sequence Homology, Amino Acid

Substances

  • Antibodies, Monoclonal
  • DNA, Complementary
  • Plant Proteins
  • Phosphotyrosine
  • MPK12 protein, Oryza sativa
  • Mitogen-Activated Protein Kinases

Associated data

  • GENBANK/AF177392