Modular regulation of cGATA-5 gene expression in the developing heart and gut

Dev Biol. 2000 Jan 1;217(1):62-76. doi: 10.1006/dbio.1999.9539.

Abstract

The evolutionarily conserved GATA-5 transcription factor is an early and persistent marker of heart and gut development in diverse vertebrate species. To search for control regions that might regulate the chicken GATA-5 (cGATA-5) gene, we assayed a set of cGATA-5/lacZ constructs in transgenic mice and found evidence for two functionally conserved control regions that regulate different facets of cGATA-5 gene expression. The more distal control region is activated in embryonic endoderm at the head-fold stage, whereas the other control region contains a regulatory module that is activated in a restricted region of endoderm following closure of the gut tube. Remarkably, the latter control region also contains a complex regulatory module that is activated in the cardiac crescent at the head-fold stage and subsequently functions in several mesodermal components of the developing heart, including the outer (epicardial) layer. We discuss these results in terms of possible contributions of epicardial-derived cells to the formation of heart valves, conduction tissue, and compact myocardium. These transgenes thus reveal, and provide a means to further analyze, transcriptional programs for several facets of heart morphogenesis and gut development.

Publication types

  • Research Support, Non-U.S. Gov't
  • Research Support, U.S. Gov't, P.H.S.

MeSH terms

  • Animals
  • Base Sequence
  • DNA
  • DNA-Binding Proteins / genetics*
  • Enhancer Elements, Genetic
  • GATA5 Transcription Factor
  • Gene Expression Regulation, Developmental*
  • Heart / embryology*
  • In Situ Hybridization
  • Intestines / embryology*
  • Mice
  • Mice, Transgenic
  • Molecular Sequence Data
  • Quail / embryology
  • Transcription Factors / genetics*

Substances

  • DNA-Binding Proteins
  • GATA5 Transcription Factor
  • Gata5 protein, mouse
  • Transcription Factors
  • DNA