Structural changes in the promoter region mediate transvection at the sn-glycerol-3-phosphate dehydrogenase gene of Drosophila melanogaster

Biochem Genet. 1999 Oct;37(9-10):301-15. doi: 10.1023/a:1018707226595.

Abstract

Trans effects at the sn-glycerol-3-phosphate dehydrogenase locus (Gpdh) of Drosophila melanogaster give rise to an increase in GPDH activity and mRNA from the wild-type allele in heterozygotes with some low-activity alleles. Either the low-activity alleles that induce the effect have a defective P-element inserted between the promoter and a downstream intronic enhancer element or the promoter region is deleted. The trans effect is pairing dependent, characteristic of transvection at some other loci. The defective P-elements that mediate transvection are located between the promoter and at least up to 6 bp downstream of the transcription start site. Transvection at Gpdh appears similar to the "enhancer action in trans" mode at the yellow locus.

MeSH terms

  • Animals
  • Base Sequence
  • Blotting, Northern
  • DNA Transposable Elements*
  • DNA-Binding Proteins / genetics
  • Drosophila Proteins
  • Drosophila melanogaster / genetics*
  • Female
  • Glycerolphosphate Dehydrogenase / genetics*
  • Glycerolphosphate Dehydrogenase / metabolism
  • Heterozygote
  • Insect Proteins / genetics*
  • Insect Proteins / metabolism
  • Male
  • Molecular Sequence Data
  • Promoter Regions, Genetic*
  • RNA, Messenger
  • Transcription, Genetic

Substances

  • DNA Transposable Elements
  • DNA-Binding Proteins
  • Drosophila Proteins
  • Insect Proteins
  • RNA, Messenger
  • z protein, Drosophila
  • Glycerolphosphate Dehydrogenase