The effect of melatonin on in vitro fertilization and embryo development in mice

J Pineal Res. 2000 Jan;28(1):48-51. doi: 10.1034/j.1600-079x.2000.280107.x.

Abstract

To examine the effect of melatonin on in vitro fertilization and embryonic development, mouse embryos after insemination in vitro were cultured in a physiological medium with or without melatonin. Melatonin increased the fertilization rate significantly at a concentration between 10(-6) and 10(-4) M (27.6 vs. 43.9 or 40.4%, P < 0.01). Furthermore, a significant increase in the rate of embryos reaching the four-cell stage (16.0 vs. 26.7%, P < 0.01), the eight-cell stage (12.1 vs. 25.8 or 23.5%, P < 0.01), and blastulation (8.9 vs. 23.5 or 17.5%, P < 0.01) was observed when the embryos were cultured in a medium containing 10(-8) or 10(-6) M melatonin. These results demonstrate that melatonin supports fertilization and early embryo development after in vitro fertilization.

MeSH terms

  • Animals
  • Blastocyst / drug effects*
  • Embryonic Development / drug effects
  • Embryonic and Fetal Development / drug effects*
  • Female
  • Fertilization in Vitro*
  • Free Radical Scavengers / pharmacology*
  • Melatonin / pharmacology*
  • Mice
  • Mice, Inbred ICR
  • Pregnancy

Substances

  • Free Radical Scavengers
  • Melatonin