Differentiation of phagocytic monocytes into lymph node dendritic cells in vivo

Immunity. 1999 Dec;11(6):753-61. doi: 10.1016/s1074-7613(00)80149-1.


We investigated the differentiation and trafficking of inflammatory monocytes that phagocytosed subcutaneously injected fluorescent microspheres. As expected, most of the monocytes became microsphere+ macrophages, which remained in subcutaneous tissue. However, about 25% of latex+ cells migrated to the T cell area of draining lymph nodes, where they expressed dendritic cell (DC)-restricted markers and high levels of costimulatory molecules. Microsphere-transporting cells were distinct from resident skin DCs, and this transport was reduced by more than 85% in monocyte-deficient osteopetrotic mice. Thus, a substantial minority of inflammatory monocytes carry phagocytosed particles to lymph nodes and differentiate into DCs.

Publication types

  • Research Support, Non-U.S. Gov't
  • Research Support, U.S. Gov't, P.H.S.

MeSH terms

  • Animals
  • Cell Differentiation
  • Dendritic Cells / classification
  • Dendritic Cells / cytology*
  • Female
  • Immunophenotyping
  • Langerhans Cells / immunology
  • Lymph Nodes / cytology*
  • Mice
  • Mice, Inbred C57BL
  • Microspheres
  • Monocytes / classification
  • Monocytes / cytology*
  • Phagocytosis
  • Skin / cytology