Expression of bcl-2 and Bax in EGFR-antisense transfected and untransfected glioblastoma cells

Anticancer Res. Sep-Oct 1999;19(5B):4167-70.


Glioblastomas are the most frequent and most malignant astrocytic gliomas. The epidermal growth factor receptor (EGFR) gene is the most frequently amplified and overexpressed in glioblastomas. The expression of bcl-2 and Bax in EGFR-antisense transfected and un-transfected glioblastoma cell line, U87E and U87V was studied by immunohistochemistry and western blotting. Our results show that the expression of Bax was stronger and bcl-2 was weaker in EGFR-antisense transfected cell line than the untransfected control. Bcl-2 and Bax genes are probably involved in the reduction of malignancy of glioblastoma cell caused by the introduction of EGFR-antisense into these tumor cells.

MeSH terms

  • Apoptosis
  • Blotting, Western
  • ErbB Receptors / genetics*
  • Glioblastoma / pathology*
  • Humans
  • Immunohistochemistry
  • Neoplasms / pathology*
  • Oligonucleotides, Antisense / metabolism
  • Proto-Oncogene Proteins / biosynthesis*
  • Proto-Oncogene Proteins c-bcl-2 / biosynthesis*
  • Transfection
  • Tumor Cells, Cultured
  • bcl-2-Associated X Protein


  • BAX protein, human
  • Oligonucleotides, Antisense
  • Proto-Oncogene Proteins
  • Proto-Oncogene Proteins c-bcl-2
  • bcl-2-Associated X Protein
  • ErbB Receptors