The Spatial Localization of T-DNA Insertions in Petunia Interphase Nuclei: Consequences for Chromosome Organization and Transgene Insertion Sites

Chromosome Res. 1999;7(8):611-23. doi: 10.1023/a:1009232002104.

Abstract

In an earlier fluorescent in-situ hybridization (FISH) study on petunia (ten Hoopen et al. 1996), we found a considerable discrepancy between the genetic map and the physical map with respect to T-DNA insertions on metaphase chromosomes. For some transgenes we found a preference to integrate near the telomeres. Here, we studied the spatial position of transgenes in interphase nuclei by FISH and 3D-confocal microscopy to elucidate a possible structural preference for the nuclear localization of transgenes. Three transgenes located near telomeres on three different metaphase chromosomes showed a much more internal distribution in interphase root meristem than the telomeres, whereas a proximal transgene appeared to be distributed in a random fashion. The results point to local differences in chromatin compacting along a chromosome. These differences might explain a preference for T-DNA insertion in distal regions of the chromosome.

Publication types

  • Research Support, Non-U.S. Gov't

MeSH terms

  • Cell Nucleus / metabolism*
  • Chromosomes*
  • DNA, Bacterial / genetics*
  • In Situ Hybridization, Fluorescence
  • Interphase*
  • Solanaceae / genetics*
  • Solanaceae / ultrastructure
  • Transgenes*

Substances

  • DNA, Bacterial
  • T-DNA