A drop-spreading technique to produce cytoplasm-free mitotic preparations from plants with small chromosomes

Chromosome Res. 1999;7(8):641-7. doi: 10.1023/a:1009288119851.


A preparation technique has been developed for plants with small chromosomes, which produces large numbers of good-quality mitotic preparations. The technique employs a hydrochloric acid treatment to hydrolyse the cytoplasm, facilitating the subsequent removal of cytoplasmic debris. The evaporative force of a methanol-based fixative is exploited to disperse the cytoplasm and to deposit the chromosomes in a single optical plane. This technique permits detailed observations of chromosome morphology and karyotyping. The mitotic preparations are also suitable for the complex analysis associated with in-situ hybridization, as in studies of genome interaction in plant hybrids.

Publication types

  • Research Support, Non-U.S. Gov't

MeSH terms

  • Chromosomes*
  • Cytoplasm
  • In Situ Hybridization, Fluorescence
  • Karyotyping
  • Mitosis*
  • Plant Cells
  • Plants / genetics*