Molecular grouping of Listeria monocytogenes based on the sequence of the inIB gene

J Med Microbiol. 2000 Jan;49(1):73-80. doi: 10.1099/0022-1317-49-1-73.


The major part of the gene inlB was sequenced in 24 strains of Listeria monocytogenes belonging to serovars 1/2a, 1/2b, 1/2c, 3b and 4b. A phylogenetic analysis based on the inlB nucleotide sequences showed that strains of serovars 1/2a and 1/2c were closely related, as well as those of serovars 1/2b and 3b. Strains sharing serovar 4b could be divided into two distinct groups. There were differences in amino-acid sequence between all serovars except between serovars 1/2b and 3b. Differences in amino-acid sequence were also seen within each of the serovars 1/2a and 4b. The data presented indicate that the inlB gene may be useful for typing purposes as an alternative or complement to serotyping.

Publication types

  • Research Support, Non-U.S. Gov't

MeSH terms

  • Amino Acid Sequence
  • Animals
  • Bacterial Proteins / chemistry
  • Bacterial Proteins / genetics*
  • Base Sequence
  • Electrophoresis, Gel, Pulsed-Field
  • Genetic Variation
  • Humans
  • Listeria monocytogenes / classification*
  • Listeria monocytogenes / genetics
  • Membrane Proteins / chemistry
  • Membrane Proteins / genetics*
  • Molecular Sequence Data
  • Phylogeny
  • Restriction Mapping


  • Bacterial Proteins
  • Membrane Proteins
  • inlB protein, Listeria monocytogenes