Aberrant Overexpression of 53BP2 mRNA in Lung Cancer Cell Lines

FEBS Lett. 2000 Jan 14;465(2-3):124-8. doi: 10.1016/s0014-5793(99)01726-3.

Abstract

The p53-binding protein 2 (53BP2) was identified as a binding protein to a tumor suppressor p53. We examined the genetic aberrations of 53BP2 gene in various human cancer cell lines. Although no gross genomic alteration or mutation of 53BP2 gene was observed, 53BP2 mRNA levels were highly variable. There was no association between the 53BP2 mRNA level and the p53 status. When we examined sensitivities of these cell lines to DNA-damaging agents including UV irradiation, X-ray irradiation and cis-diamine-dichloroplatinum (CDDP), we found that higher 53BP2 mRNA expression was correlated with the sensitivity to these agents.

MeSH terms

  • Apoptosis Regulatory Proteins
  • Carrier Proteins / genetics*
  • Cisplatin / pharmacology
  • DNA Damage
  • DNA, Complementary
  • Lung Neoplasms / genetics*
  • RNA, Messenger / genetics*
  • Tumor Cells, Cultured
  • Ultraviolet Rays
  • X-Rays

Substances

  • Apoptosis Regulatory Proteins
  • Carrier Proteins
  • DNA, Complementary
  • RNA, Messenger
  • TP53BP2 protein, human
  • Cisplatin