Potential role for excretory-secretory forms of glutathione-S-transferase (GST) in Fasciola hepatica

Parasitology. 1999 Dec;119 ( Pt 6):627-33. doi: 10.1017/s003118209900517x.

Abstract

The excretory-secretory antigen of Fasciola hepatica (ESA) is involved in the suppressive phenomena of cellular immune responses in rats. The ESA can depress the proliferative response of spleen mononuclear cells and inhibit nitric oxide (NO) production by peritoneal cells. In the present study we identified ESA proteins of ca 24 kDa, which shared significant sequence homology to glutathione-S-transferase (GST) obtained from homogenates of F. hepatica adults, other helminths and different mammals. When the dimeric form of these proteins ca 48 kDa was cultured with rat spleen cells, a significant decrease of proliferative response to Con A was detected, starting from 20 micrograms/ml of ESA protein (P < 0.03). We also observed a significant inhibition of nitrite production by incubation with the dimeric form in normal peritoneal macrophages (P < 0.04). These results indicated that the GST secreted by the parasite could be involved in evasion of the parasite from the host immune response.

Publication types

  • Research Support, Non-U.S. Gov't

MeSH terms

  • Amino Acid Sequence
  • Animals
  • Antigens, Helminth / immunology*
  • Concanavalin A / pharmacology
  • Fasciola hepatica / enzymology*
  • Fasciola hepatica / immunology
  • Glutathione Transferase / chemistry*
  • Glutathione Transferase / immunology*
  • Glutathione Transferase / metabolism
  • Lymphocyte Activation
  • Macrophages, Peritoneal / immunology
  • Macrophages, Peritoneal / metabolism
  • Male
  • Molecular Sequence Data
  • Nitric Oxide / biosynthesis
  • Rats
  • Rats, Wistar
  • Spleen / cytology
  • Spleen / immunology

Substances

  • Antigens, Helminth
  • Concanavalin A
  • Nitric Oxide
  • Glutathione Transferase