Genetic Analysis of BRCA1 Function in a Defined Tumor Cell Line

Mol Cell. 1999 Dec;4(6):1093-9. doi: 10.1016/s1097-2765(00)80238-5.

Abstract

Retrovirally expressed, wild-type BRCA1 decreased the gamma radiation (IR) sensitivity and increased the efficiency of double-strand DNA break repair (DSBR) of the BRCA1-/- human breast cancer line, HCC1937. It also reduced its susceptibility to DSB generation by IR. By contrast, multiple, clinically validated, missense mutant BRCA1 products were nonfunctional in these assays. These data constitute the basis for a BRCA1 functional assay and suggest that efficient repair of double-strand DNA breaks is linked to BRCA1 tumor suppression function.

Publication types

  • Research Support, Non-U.S. Gov't
  • Research Support, U.S. Gov't, Non-P.H.S.
  • Research Support, U.S. Gov't, P.H.S.

MeSH terms

  • BRCA1 Protein / genetics*
  • Breast Neoplasms / genetics*
  • Breast Neoplasms / radiotherapy
  • DNA Repair / genetics
  • DNA Repair / radiation effects
  • Female
  • Gene Expression Regulation, Neoplastic*
  • Gene Transfer Techniques
  • Genetic Vectors
  • Humans
  • Radiation Tolerance / genetics
  • Retroviridae
  • Tumor Cells, Cultured

Substances

  • BRCA1 Protein