Leukocyte adhesion molecule expression and T cell naïve/memory status following isoproterenol infusion

J Neuroimmunol. 2000 Jan 24;102(2):137-44. doi: 10.1016/s0165-5728(99)00180-0.


This study examined adhesion molecules on peripheral leukocytes following a 30-min infusion of the beta-adrenergic agonist isoproterenol in 23 healthy subjects. In response to isoproterenol, the number of CD8 +CD62L- T cells and both CD62L+ and CD62L-natural killer (NK) (CD3 CD16+ 56+) cells increased markedly in circulation (p < 0.001). In addition, the surface density of CD62L was significantly lower on both CD8+ and CD4+ T cells (p < 0.001). Plasma levels of soluble CD62L remained unchanged, arguing against an isoproterenol-induced shedding of L-selectin. In contrast to CD62L, the surface density of the beta2 integrin LFA-1 (CD11a) was higher on circulating lymphocytes (p < 0.001) (but not monocytes or lymphocytes) post-infusion. Isoproterenol also led to a mobilization of memory/activated CD8+CD29high T cells (p < 0.01), but had no significant effect on the number of circulating CD8+ CD45RA+ CD62L+ naïve T cells. beta blockade with the non-specific antagonist propranolol eliminated these isoproterenol-induced effects.

Publication types

  • Research Support, U.S. Gov't, P.H.S.

MeSH terms

  • Adrenergic beta-Agonists / pharmacology*
  • Adult
  • Blood Cells / immunology
  • CD18 Antigens / analysis
  • CD4-Positive T-Lymphocytes / immunology
  • CD8-Positive T-Lymphocytes / immunology
  • Cell Adhesion Molecules / metabolism*
  • Cell Membrane / immunology
  • Female
  • Humans
  • Immunologic Memory
  • Integrin beta1 / analysis
  • Intercellular Adhesion Molecule-1 / analysis
  • Isoproterenol / pharmacology*
  • L-Selectin / analysis
  • Lymphocyte Function-Associated Antigen-1 / analysis
  • Lymphocytes / immunology
  • Male
  • Middle Aged
  • T-Lymphocytes / immunology
  • T-Lymphocytes / physiology*


  • Adrenergic beta-Agonists
  • CD18 Antigens
  • Cell Adhesion Molecules
  • Integrin beta1
  • Lymphocyte Function-Associated Antigen-1
  • Intercellular Adhesion Molecule-1
  • L-Selectin
  • Isoproterenol