Plant organ size control: AINTEGUMENTA regulates growth and cell numbers during organogenesis

Proc Natl Acad Sci U S A. 2000 Jan 18;97(2):942-7. doi: 10.1073/pnas.97.2.942.

Abstract

The control of cell proliferation during organogenesis plays an important role in initiation, growth, and acquisition of the intrinsic size of organs in higher plants. To understand the developmental mechanism that controls intrinsic organ size by regulating the number and extent of cell division during organogenesis, we examined the function of the Arabidopsis regulatory gene AINTEGUMENATA (ANT). Previous observations revealed that ANT regulates cell division in integuments during ovule development and is necessary for floral organ growth. Here we show that ANT controls plant organ cell number and organ size throughout shoot development. Loss of ANT function reduces the size of all lateral shoot organs by decreasing cell number. Conversely, gain of ANT function, via ectopic expression of a 35S::ANT transgene, enlarges embryonic and all shoot organs without altering superficial morphology by increasing cell number in both Arabidopsis and tobacco plants. This hyperplasia results from an extended period of cell proliferation and organ growth. Furthermore, cells ectopically expressing ANT in fully differentiated organs exhibit neoplastic activity by producing calli and adventitious roots and shoots. Based on these results, we propose that ANT regulates cell proliferation and organ growth by maintaining the meristematic competence of cells during organogenesis.

Publication types

  • Research Support, U.S. Gov't, Non-P.H.S.

MeSH terms

  • Arabidopsis / cytology
  • Arabidopsis / genetics
  • Arabidopsis / growth & development*
  • Arabidopsis Proteins*
  • Cell Count
  • Cell Division / physiology
  • Cellular Senescence
  • Cyclin D3
  • Cyclins / genetics
  • Gene Expression Regulation, Developmental
  • Mutation
  • Plant Leaves / genetics
  • Plant Leaves / growth & development
  • Plant Proteins / genetics
  • Plant Proteins / physiology*
  • Plant Structures / cytology
  • Plant Structures / growth & development*
  • Plant Structures / ultrastructure
  • Plants, Genetically Modified / cytology
  • Plants, Genetically Modified / growth & development
  • Plants, Toxic
  • RNA, Messenger / genetics
  • RNA, Messenger / metabolism
  • Time Factors
  • Tobacco / genetics
  • Tobacco / growth & development
  • Transcription Factors / genetics
  • Transcription Factors / physiology*

Substances

  • AINTEGUMENTA protein, Arabidopsis
  • Arabidopsis Proteins
  • Cyclin D3
  • Cyclins
  • Plant Proteins
  • RNA, Messenger
  • Transcription Factors