Acquisition of chloramphenicol resistance by the linearization and integration of the entire staphylococcal plasmid pC194 into the chromosome of Streptococcus pneumoniae

C A Widdowson; P V Adrian; K P Klugman

doi:10.1128/AAC.44.2.393-395.2000

Acquisition of chloramphenicol resistance by the linearization and integration of the entire staphylococcal plasmid pC194 into the chromosome of Streptococcus pneumoniae

Antimicrob Agents Chemother. 2000 Feb;44(2):393-5. doi: 10.1128/AAC.44.2.393-395.2000.

Authors

C A Widdowson¹, P V Adrian, K P Klugman

Affiliation

¹ Medical Research Council, South African Institute for Medical Research, WITS Pneumococcal Diseases Research Unit, Johannesburg, South Africa.

Abstract

Chloramphenicol resistance in Streptococcus pneumoniae was associated with cat, which has 100% identity with cat(pC194) from Staphylococcus aureus. Inverse PCR with primers specific for pC194 confirmed that in some isolates the entire staphylococcal plasmid was present in the S. pneumoniae chromosome, with linearization having occurred between cat(pC194) and the origin of replication.

MeSH terms

Base Sequence
Chloramphenicol O-Acetyltransferase / analysis
Chloramphenicol O-Acetyltransferase / genetics
Chloramphenicol Resistance / genetics*
Chromosomes, Bacterial
DNA, Bacterial / analysis
Humans
Molecular Sequence Data
Plasmids / genetics
Polymerase Chain Reaction
Replication Origin
Sequence Analysis
Streptococcus pneumoniae / enzymology
Streptococcus pneumoniae / genetics*

Substances

DNA, Bacterial
Chloramphenicol O-Acetyltransferase