Comparative evaluation of sensitivity of RNA-polyacrylamide gel electrophoresis and dot immunobinding assay for detection of bluetongue virus in cell culture

G Prasad; Minakshi

Comparative evaluation of sensitivity of RNA-polyacrylamide gel electrophoresis and dot immunobinding assay for detection of bluetongue virus in cell culture

Indian J Exp Biol. 1999 Feb;37(2):157-60.

Authors

G Prasad¹, Minakshi

Affiliation

¹ Department of Veterinary Microbiology, CCS Haryana Agricultural University, Hisar, India.

PMID: 10641136

Abstract

Bluetongue virus serotype 1 (Avikanagar isolate) was grown in BHK-21 cell line and titrated. The titre of the virus in BHK-21 cell line was 10(6) TCID50/ml. RNA-polyacrylamide gel electrophoresis (RNA-PAGE) and dot immunobinding assay (DIA) were performed on 10-fold serial dilutions of the sonicated cell culture material. The results indicated that the minimum limit of detection of the virus by RNA-PAGE and DIA was 10(5) TCID50/ml.

Publication types

Comparative Study
Research Support, Non-U.S. Gov't

MeSH terms

Animals
Bluetongue virus / classification
Bluetongue virus / immunology
Bluetongue virus / isolation & purification*
Cell Line
Cricetinae
Electrophoresis, Polyacrylamide Gel / methods
Electrophoresis, Polyacrylamide Gel / statistics & numerical data
Immunoblotting / statistics & numerical data
RNA, Viral / isolation & purification
Sensitivity and Specificity

Substances

RNA, Viral