In this study, we demonstrated bcl-2 expression in the rabbit brain using in situ hybridization and compared the results with the studies done on the other animals. Although the bcl-2 expressions of the rabbit were generally similar to those of the other animals, some differences were also found; the cells in molecular and Purkinje cell layers of the cerebellum, which were bcl-2 negative in the others, showed intense bcl-2 positive signals, while the ependymal cells, arachnoid villi and granular layer, which were positive in the other animals, were not well stained with bcl-2 riboprobes in the rabbit brain. Our findings clearly showed the similarities and differences of rabbit bcl-2 mRNA as compared with the other species for the first time, and provided the basis of further study on the properties of bcl-2 in this species.