Adaptation to high-fat diet reduces inhibition of gastric emptying by CCK and intestinal oleate

Am J Physiol Regul Integr Comp Physiol. 2000 Jan;278(1):R166-70. doi: 10.1152/ajpregu.2000.278.1.R166.


Rats maintained on low-fat (LF) or high-fat (HF) diets were fitted with gastric cannulas and duodenal catheters. Intraperitoneal injection of 0.250-2.0 microg/kg cholecystokinin (CCK) significantly inhibited gastric emptying of a 5-ml NaCl load in LF rats by 26.2-55. 1% compared with emptying after vehicle injection. By contrast, CCK-induced inhibition of gastric emptying was significantly less in HF rats given the same CCK doses (10.0-31.7% inhibition over the same CCK dose range). A 20-min intraduodenal infusion of oleate (0.03 or 0.06 kcal/ml) also resulted in significant inhibition of gastric emptying in LF rats (24 and 89%, respectively). Oleate-induced inhibition of gastric emptying was significantly attenuated in rats maintained on the HF diet (2 and 56%, respectively). Unlike CCK injections or oleate infusion, intraduodenal maltotriose infusion inhibited gastric emptying to a similar degree in LF and HF rats (77 and 78%, respectively). These results indicate that feeding HF diets diminishes the enterogastric inhibition of gastric emptying by intestinal oleate and diminishes the ability of CCK to inhibit gastric emptying.

Publication types

  • Research Support, U.S. Gov't, P.H.S.

MeSH terms

  • Adaptation, Physiological*
  • Animals
  • Cholecystokinin / antagonists & inhibitors
  • Cholecystokinin / pharmacology*
  • Dietary Fats / administration & dosage*
  • Dietary Fats / pharmacology
  • Gastric Emptying / drug effects*
  • Intestines / physiology*
  • Male
  • Oleic Acid / antagonists & inhibitors
  • Oleic Acid / pharmacology*
  • Rats
  • Rats, Sprague-Dawley
  • Sodium Chloride / pharmacology


  • Dietary Fats
  • Oleic Acid
  • Sodium Chloride
  • Cholecystokinin