Role of lumican in the corneal epithelium during wound healing

J Biol Chem. 2000 Jan 28;275(4):2607-12. doi: 10.1074/jbc.275.4.2607.


Lumican regulates collagenous matrix assembly as a keratan sulfate proteoglycan in the cornea and is also present in the connective tissues of other organs and embryonic corneal stroma as a glycoprotein. In normal unwounded cornea, lumican is expressed by stromal keratocytes. Our data show that injured mouse corneal epithelium ectopically and transiently expresses lumican during the early phase of wound healing, suggesting a potential lumican functionality unrelated to regulation of collagen fibrillogenesis, e. g. modulation of epithelial cell adhesion or migration. An anti-lumican antibody was found to retard corneal epithelial wound healing in cultured mouse eyes. Healing of a corneal epithelial injury in Lum(-/-) mice was significantly delayed compared with Lum(+/-) mice. These observations indicate that lumican expressed in injured epithelium may modulate cell behavior such as adhesion or migration, thus contributing to corneal epithelial wound healing.

Publication types

  • Research Support, Non-U.S. Gov't
  • Research Support, U.S. Gov't, P.H.S.

MeSH terms

  • Amino Acid Sequence
  • Animals
  • Antibodies / immunology
  • Chondroitin Sulfate Proteoglycans / genetics
  • Chondroitin Sulfate Proteoglycans / immunology
  • Chondroitin Sulfate Proteoglycans / physiology*
  • Epithelium, Corneal / physiopathology*
  • Immunohistochemistry
  • In Situ Hybridization
  • Keratan Sulfate / genetics
  • Keratan Sulfate / immunology
  • Keratan Sulfate / physiology*
  • Lumican
  • Mice
  • Mice, Inbred C57BL
  • Molecular Sequence Data
  • Phenotype
  • RNA, Messenger / genetics
  • Wound Healing*


  • Antibodies
  • Chondroitin Sulfate Proteoglycans
  • Lum protein, mouse
  • Lumican
  • RNA, Messenger
  • Keratan Sulfate