Expression of angiogenic growth factors in paragangliomas

Laryngoscope. 2000 Jan;110(1):161-7. doi: 10.1097/00005537-200001000-00029.

Abstract

Objective/hypothesis: To determine if angiogenic growth factors including vascular endothelial growth factor (VEGF) and platelet-derived endothelial cell growth factor (PD-ECGF) are expressed in human paragangliomas.

Study design: A histopathologic and molecular examination of paraganglioma specimens obtained from surgical cases or retrieved from the Pathology Department of the Massachusetts Eye and Ear Infirmary.

Methods: Fresh tumor or archival, paraffin-embedded paraganglioma specimens were analyzed by immunohistochemistry, Western blotting, and ELISA.

Results: Positive immunohistochemical staining for VEGF was observed in five of nine surgical specimens and in six of eight archival specimens (11/17, or 65%). PD-ECGF immunoreactivity was detected in four of five surgical specimens and six of eight archival specimens (10/13, or 77%). The presence of PD-ECGF was confirmed by Western blot assay and ELISA confirmed the presence of VEGF in tumor extract.

Conclusions: Both VEGF and PD-ECGF are expressed in paragangliomas and may contribute to the extreme vascularity of these tumors. Key Words. Vascular endothelial growth factor, platelet-derived, endothelial cell growth factor, hypoxia, tumor vasculature.

Publication types

  • Research Support, Non-U.S. Gov't
  • Research Support, U.S. Gov't, P.H.S.

MeSH terms

  • Angiogenesis Inducing Agents / analysis
  • Angiogenesis Inducing Agents / metabolism*
  • Aortic Bodies / chemistry
  • Aortic Bodies / metabolism
  • Blotting, Western / methods
  • Carotid Body Tumor / chemistry
  • Carotid Body Tumor / genetics
  • Carotid Body Tumor / metabolism
  • Endothelial Growth Factors / analysis
  • Endothelial Growth Factors / metabolism
  • Enzyme-Linked Immunosorbent Assay / methods
  • Gene Expression Regulation, Neoplastic / physiology*
  • Glomus Jugulare Tumor / chemistry
  • Glomus Jugulare Tumor / genetics
  • Glomus Jugulare Tumor / metabolism
  • Humans
  • Immunohistochemistry
  • Lymphokines / analysis
  • Lymphokines / metabolism
  • Paraganglia, Nonchromaffin / chemistry
  • Paraganglia, Nonchromaffin / metabolism
  • Paraganglioma, Extra-Adrenal / chemistry
  • Paraganglioma, Extra-Adrenal / genetics*
  • Paraganglioma, Extra-Adrenal / metabolism
  • Protein Isoforms
  • Thymidine Phosphorylase / analysis
  • Thymidine Phosphorylase / metabolism
  • Vascular Endothelial Growth Factor A
  • Vascular Endothelial Growth Factors

Substances

  • Angiogenesis Inducing Agents
  • Endothelial Growth Factors
  • Lymphokines
  • Protein Isoforms
  • Vascular Endothelial Growth Factor A
  • Vascular Endothelial Growth Factors
  • Thymidine Phosphorylase