Abstract
The effect of growth in 2xYT medium on catabolite repression control in Pseudomonas putida has been investigated using the bkd operon, encoding branched-chain keto acid dehydrogenase. Crc (catabolite repression control protein) was shown to be responsible for repression of bkd operon transcription in 2xYT. BkdR levels were elevated in a P. putida crc mutant, but bkdR transcript levels were the same in both wild type and crc mutant. This suggests that the mechanism of catabolite repression control in rich media by Crc involves posttranscriptional regulation of the bkdR message.
Publication types
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Research Support, Non-U.S. Gov't
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Research Support, U.S. Gov't, P.H.S.
MeSH terms
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3-Methyl-2-Oxobutanoate Dehydrogenase (Lipoamide)
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Bacterial Proteins*
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Culture Media
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DNA-Binding Proteins / genetics*
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DNA-Binding Proteins / metabolism
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Gene Expression Regulation, Bacterial*
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Genes, Regulator
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Ketone Oxidoreductases / genetics*
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Ketone Oxidoreductases / metabolism
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Leucine-Responsive Regulatory Protein
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Multienzyme Complexes / genetics*
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Multienzyme Complexes / metabolism
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Pseudomonas putida / genetics
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Pseudomonas putida / growth & development*
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Pseudomonas putida / metabolism
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RNA Processing, Post-Transcriptional
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RNA, Messenger / metabolism
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Repressor Proteins / genetics*
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Repressor Proteins / metabolism
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Transcription Factors*
Substances
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Bacterial Proteins
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Culture Media
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DNA-Binding Proteins
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Multienzyme Complexes
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RNA, Messenger
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Repressor Proteins
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Transcription Factors
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crc protein, Pseudomonas
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Leucine-Responsive Regulatory Protein
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Ketone Oxidoreductases
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3-Methyl-2-Oxobutanoate Dehydrogenase (Lipoamide)