Molecular cloning and functional expression of Ca(v)3.1c, a T-type calcium channel from human brain

FEBS Lett. 2000 Jan 21;466(1):54-8. doi: 10.1016/s0014-5793(99)01756-1.


Low voltage-activated T-type calcium channels are encoded by a family of at least three genes, with additional diversity created by alternative splicing. This study describes the cloning of the human brain alpha1G, which is a novel isoform, Ca(v)3.1c. Comparison of this sequence to genomic sequences deposited in the GenBank allowed us to identify the intron/exon boundaries of the human CACNA1G gene. A full-length cDNA was constructed, then used to generate a stably-transfected mammalian cell line. The resulting currents were analyzed for their voltage- and time-dependent properties. These properties identify this gene as encoding a T-type Ca(2+) channel.

Publication types

  • Research Support, Non-U.S. Gov't
  • Research Support, U.S. Gov't, P.H.S.

MeSH terms

  • Alternative Splicing
  • Amino Acid Sequence
  • Animals
  • Base Sequence
  • Brain / metabolism*
  • Calcium Channels, T-Type / genetics*
  • Calcium Channels, T-Type / metabolism*
  • Cell Line
  • Cloning, Molecular
  • DNA Primers / genetics
  • DNA, Complementary / genetics
  • Exons
  • Gene Expression
  • Humans
  • Introns
  • Kinetics
  • Membrane Potentials
  • Molecular Sequence Data
  • Rats
  • Transfection


  • CACNA1G protein, human
  • Cacna1g protein, rat
  • Calcium Channels, T-Type
  • DNA Primers
  • DNA, Complementary