Cloning, expression and characterization of a novel four EF-hand Ca(2+)-binding protein from olive pollen with allergenic activity

FEBS Lett. 2000 Jan 21;466(1):192-6. doi: 10.1016/s0014-5793(99)01790-1.


A novel allergenic member of the family of Ca(2+)-binding proteins has been cloned from olive tree pollen. The isolated DNA codes for a protein of 171 amino acid residues, which displays four EF-hand sequence motifs. The encoded protein was overproduced in Escherichia coli and purified. The protein (18¿ omitted¿795 Da), which binds Ca(2+) and IgE antibodies from patients allergic to olive pollen, undergoes Ca(2+)-dependent conformational changes. It is retained on a phenyl-Sepharose column, which indicates the existence of regulatory EF-hand domains. This fact suggests its involvement in Ca(2+)-dependent signal transduction events of the pollen grain. This allergen could be considered as a member of a new subfamily of EF-hand Ca(2+)-binding proteins since it displays a low amino acid sequence similarity with the so far known proteins.

Publication types

  • Research Support, Non-U.S. Gov't

MeSH terms

  • Allergens / genetics*
  • Amino Acid Sequence
  • Base Sequence
  • Calcium-Binding Proteins / chemistry
  • Calcium-Binding Proteins / genetics*
  • Calcium-Binding Proteins / immunology
  • Cloning, Molecular
  • DNA, Complementary / genetics
  • Escherichia coli
  • Molecular Sequence Data
  • Molecular Weight
  • Plant Proteins / chemistry
  • Plant Proteins / genetics*
  • Plant Proteins / immunology
  • Pollen / genetics*
  • Pollen / immunology
  • Recombinant Proteins / chemistry
  • Recombinant Proteins / genetics
  • Recombinant Proteins / immunology
  • Sequence Homology, Amino Acid
  • Trees / genetics
  • Trees / immunology


  • Allergens
  • Calcium-Binding Proteins
  • DNA, Complementary
  • Plant Proteins
  • Recombinant Proteins

Associated data

  • GENBANK/AF078679