Localization of factor VIII-related antigen in the endothelium of the chick embryo chorioallantoic membrane

D Ribatti; B Nico; A Vacca; L Roncali

doi:10.1007/s004180050427

Localization of factor VIII-related antigen in the endothelium of the chick embryo chorioallantoic membrane

Histochem Cell Biol. 1999 Dec;112(6):447-50. doi: 10.1007/s004180050427.

Authors

D Ribatti¹, B Nico, A Vacca, L Roncali

Affiliation

¹ Institute of Human Anatomy, Histology and Embryology, University of Bari Medical School, Italy. ribatti@anatomia.uniba.it

PMID: 10651095
DOI: 10.1007/s004180050427

Abstract

In this study, by using a polyclonal antibody against factor VIII-related antigen (FVIII-RA), we have examined the expression of FVIII-RA in the blood and lymphatic vessels of the chick embryo chorioallantoic membrane (CAM). The antibody marked the endothelium of blood and lymphatic vessels starting from day 8 of incubation and the cytoplasm of the allantoic epithelial cells. The application of this antibody may be useful for quantifying neovascularization in response to various angiogenic stimuli applied to the CAM.

Publication types

Research Support, Non-U.S. Gov't

MeSH terms

Allantois / metabolism*
Animals
Antibodies
Chick Embryo
Chorion / metabolism*
Endothelium, Lymphatic / metabolism
Endothelium, Vascular / metabolism
Immunohistochemistry
Neovascularization, Physiologic
Rabbits
Time Factors
von Willebrand Factor / immunology
von Willebrand Factor / metabolism*

Substances

Antibodies
von Willebrand Factor