Convergence between CD98 and integrin-mediated T-lymphocyte co-stimulation

Immunology. 2000 Jan;99(1):62-8. doi: 10.1046/j.1365-2567.2000.00953.x.

Abstract

CD98 is a widely expressed cell surface heterodimeric glycoprotein, which is rapidly up-regulated upon activation of T lymphocytes. Monoclonal antibody (mAb) 80A10 recognizes an epitope on CD98 and in combination with CD3 antibody causes proliferation of peripheral blood T lymphocytes. CD98 co-stimulatory activity, mediated by either mAb 80A10 or 4F2, a well-characterized CD98-specific mAb, is blocked in the presence of the soluble beta1 integrin antibody 18D3. Previously we have reported that co-stimulatory activity of antibodies to integrins alpha4beta1, alpha5beta1, alphaLbeta2 and alpha4beta7 is inhibited by 18D3, whereas co-stimulation mediated by non-integrins was unaffected. Thus the non-integrin CD98 is uniquely sensitive to the inhibitory effects of beta1 integrin-blocking antibodies, which may reflect convergent signalling mechanisms between integrins and CD98. This is consistent with recent reports suggesting that CD98 may regulate integrin-mediated adhesive events.

Publication types

  • Research Support, Non-U.S. Gov't
  • Research Support, U.S. Gov't, P.H.S.

MeSH terms

  • Antibodies, Monoclonal / immunology
  • Antibodies, Monoclonal / isolation & purification
  • Antigens, CD / immunology*
  • CD3 Complex / immunology*
  • Carrier Proteins / immunology*
  • Cell Line
  • Flow Cytometry
  • Fusion Regulatory Protein-1
  • Humans
  • Integrin alpha4beta1
  • Integrins / immunology*
  • Ionomycin / pharmacology
  • Ionophores / pharmacology
  • Lymphocyte Activation*
  • Precipitin Tests
  • Protein Binding / drug effects
  • Receptors, Lymphocyte Homing / immunology
  • Signal Transduction*
  • T-Lymphocytes / immunology*
  • Tetradecanoylphorbol Acetate / pharmacology

Substances

  • Antibodies, Monoclonal
  • Antigens, CD
  • CD3 Complex
  • Carrier Proteins
  • Fusion Regulatory Protein-1
  • Integrin alpha4beta1
  • Integrins
  • Ionophores
  • Receptors, Lymphocyte Homing
  • Ionomycin
  • Tetradecanoylphorbol Acetate