Comamonas Acidovorans Strain MC1: A New Isolate Capable of Degrading the Chiral Herbicides Dichlorprop and Mecoprop and the Herbicides 2,4-D and MCPA

Microbiol Res. 1999 Dec;154(3):241-6. doi: 10.1016/S0944-5013(99)80021-4.

Abstract

A gram-negative prototrophic bacterial species, strain MC1, was isolated from the vicinity of herbicide-contaminated building rubble and identified by 16S rDNA sequence analysis, its physiological properties, GC content, and fatty acid composition as Comamonas acidovorans. This strain displays activity for the productive degradation of the two enantiomers of dichlorprop [(RS)-2-(2,4-dichlorophenoxy-)propionate; (RS)-2,4-DP] and mecoprop [(RS)-2-(4-chloro-2-methyl-) phenoxypropionate; (RS)-MCPP] in addition phenoxyacetate herbicides, i.e. 2,4-dichlorophenoxyacetate (2,4-D) and 4-chloro-2-methylphenoxyacetate (MCPA), and various chlorophenols were utilized. Rates amounted to 1.2 mmoles/h g dry mass (2,4-D) and 2.7 mmoles/h g dry mass [(RS)-2,4-DP]. Degradation of (RS)-2,4-DP was not inhibited up to concentrations of 500 mg/l, nor of 2,4-D up to 200 mg/l. The optimum pH value of (RS)-2,4-DP degradation was around 8. The application of respective primers for PCR amplification revealed the presence of tfdB and tfdC genes.

MeSH terms

  • 2,4-Dichlorophenoxyacetic Acid / analogs & derivatives*
  • 2,4-Dichlorophenoxyacetic Acid / metabolism*
  • 2-Methyl-4-chlorophenoxyacetic Acid / analogs & derivatives*
  • 2-Methyl-4-chlorophenoxyacetic Acid / metabolism*
  • Base Composition
  • Biodegradation, Environmental
  • Chlorophenols / metabolism
  • DNA, Bacterial / genetics
  • Delftia acidovorans / classification
  • Delftia acidovorans / genetics
  • Delftia acidovorans / isolation & purification*
  • Delftia acidovorans / metabolism*
  • Herbicides / metabolism*
  • Hydrogen-Ion Concentration
  • Phylogeny
  • RNA, Ribosomal, 16S / analysis
  • Soil Microbiology

Substances

  • Chlorophenols
  • DNA, Bacterial
  • Herbicides
  • RNA, Ribosomal, 16S
  • 2,4-Dichlorophenoxyacetic Acid
  • mecoprop
  • 2-Methyl-4-chlorophenoxyacetic Acid
  • dichlorprop

Associated data

  • GENBANK/AF077917
  • GENBANK/AF149849