Levels of 8-hydroxydeoxyguanosine as a marker of DNA damage in human leukocytes

Free Radic Biol Med. 2000 Jan 1;28(1):13-7. doi: 10.1016/s0891-5849(99)00194-x.

Abstract

We measured 8-hydroxy-2-deoxyguanosine (8-OHdG) levels in human leukocytes from healthy donors to evaluate oxidative DNA damage and its correlation with smoking, physical exercise, and alcohol consumption. A significant increase in oxidative DNA damage was induced by cigarette smoke, with the mean level of 8-OHdG being significantly higher in smokers (33.1 +/- 10.6 per 10(6) 2-deoxyguanosine (dG) [mean +/- SE], n = 16) compared with nonsmokers (15.3 +/- 1.8 per 10(6) dG, n = 31) and former smokers (17.8 +/- 1.5 per 10(6) dG, n = 9). The highest values were observed after smoking more than 10 cigarettes per day (41.8 +/- 17.1 per 10(6) dG, n = 9). A large interindividual variation in 8-OHdG levels was observed in all analyzed groups. We also observed a correlation between 8-OHdG levels and age in nonsmokers and former smokers. Neither frequency of physical exercise nor alcohol drinking significantly modified 8-OHdG levels in leukocytes.

Publication types

  • Research Support, Non-U.S. Gov't

MeSH terms

  • Adolescent
  • Adult
  • Alcohol Drinking / blood
  • Biomarkers
  • Body Mass Index
  • DNA Damage*
  • Exercise
  • Female
  • Guanosine / analogs & derivatives*
  • Guanosine / blood
  • Humans
  • Leukocytes / chemistry*
  • Male
  • Middle Aged
  • Oxidative Stress
  • Smoking / blood
  • Smoking Cessation

Substances

  • Biomarkers
  • Guanosine
  • 8-hydroxyguanosine