Blood perfusion of subcutaneous tumours in mice following the application of low-level direct electric current

Adv Exp Med Biol. 1999;471:497-506. doi: 10.1007/978-1-4615-4717-4_59.


Electrotherapy with low-level direct electric current has been proved to be an effective local treatment of solid tumours. In the presented study an attempt was made to evaluate the effect of a single treatment with electrotherapy on blood perfusion of solid subcutaneous fibrosarcoma Sa-1 tumours in A/J mice. The tissue-staining method with Patent blue-violet dye, the rubidium extraction technique, and the noninvasive near-infrared spectroscopy method were used for this purpose. Results of all methods indicate that perfusion and subsequently oxygenation of tumours were reduced due to application of electrotherapy.

Publication types

  • Research Support, Non-U.S. Gov't

MeSH terms

  • Animals
  • Coloring Agents
  • Electric Stimulation Therapy* / methods
  • Female
  • Fibrosarcoma / blood*
  • Fibrosarcoma / metabolism
  • Fibrosarcoma / therapy
  • Male
  • Mice
  • Mice, Inbred A
  • Rosaniline Dyes
  • Rubidium
  • Skin Neoplasms / blood*
  • Skin Neoplasms / metabolism
  • Skin Neoplasms / therapy
  • Spectroscopy, Near-Infrared
  • Staining and Labeling / methods


  • Coloring Agents
  • Rosaniline Dyes
  • sulfan blue
  • Rubidium