Small, Stable Shuttle Vectors Based on the Minimal pVS1 Replicon for Use in Gram-Negative, Plant-Associated Bacteria

Mol Plant Microbe Interact. 2000 Feb;13(2):232-7. doi: 10.1094/MPMI.2000.13.2.232.

Abstract

The minimal replicon of the Pseudomonas plasmid pVS1 was genetically defined and combined with the Escherichia coli p15A replicon, to provide a series of new, oligocopy cloning vectors (5.3 to 8.3 kb). Recombinant plasmids derived from these vectors were stable in growing and nongrowing cells of root-colonizing P. fluorescens strains incubated under different environmental conditions for more than 1 month.

Publication types

  • Research Support, Non-U.S. Gov't

MeSH terms

  • Amino Acid Sequence
  • Base Sequence
  • DNA, Recombinant / genetics
  • Escherichia coli / genetics
  • Genetic Vectors*
  • Molecular Sequence Data
  • Plants / microbiology*
  • Plasmids / genetics
  • Pseudomonas fluorescens / genetics*
  • Replicon / genetics*
  • Sequence Homology, Amino Acid

Substances

  • DNA, Recombinant

Associated data

  • GENBANK/AF118810
  • GENBANK/AF118811
  • GENBANK/AF118812
  • GENBANK/AF133828
  • GENBANK/AF133829
  • GENBANK/AF133830
  • GENBANK/AF133831
  • GENBANK/U10456
  • GENBANK/U10457
  • GENBANK/U10458
  • GENBANK/U10459
  • GENBANK/U10460
  • GENBANK/U10461
  • GENBANK/U10462
  • GENBANK/U10463
  • GENBANK/U10487
  • GENBANK/U10488
  • GENBANK/U10489
  • GENBANK/U10490
  • GENBANK/U10491