Controlled expression of tagged proteins in Drosophila using a new modular P-element vector system

Mol Gen Genet. 2000 Jan;262(6):916-20. doi: 10.1007/pl00008659.


We have developed a new modular vector system that facilitates the combination of various DNA fragments as functional modules for P element-mediated transformation of Drosophila melanogaster. The basic vector pP¿GS¿ contains unique sites for 17 restriction enzymes, including three 8-bp cutters, that allow one to combine various promoter elements, cDNAs and genomic DNA fragments, as well as protein tags and selectable marker genes, for a wide spectrum of transgene analyses. With this new vector system we analysed the chromosomal distribution of the Drosophila SU(VAR)3-9 protein tagged with EGFP, using hsp70-cDNA and genomic Su(var)3-9 constructs. We found preferential association of the tagged SU(VAR)3-9 with centric heterochromatin.

Publication types

  • Research Support, Non-U.S. Gov't

MeSH terms

  • Animals
  • Animals, Genetically Modified
  • Base Sequence
  • DNA Primers / genetics
  • Drosophila melanogaster / genetics*
  • Gene Expression
  • Genetic Vectors*
  • Green Fluorescent Proteins
  • Heterochromatin / genetics
  • Luminescent Proteins / genetics
  • Promoter Regions, Genetic
  • Recombinant Fusion Proteins / genetics
  • Transformation, Genetic*


  • DNA Primers
  • Heterochromatin
  • Luminescent Proteins
  • Recombinant Fusion Proteins
  • Green Fluorescent Proteins