Positive selection from newly formed to marginal zone B cells depends on the rate of clonal production, CD19, and btk

Immunity. 2000 Jan;12(1):39-49. doi: 10.1016/s1074-7613(00)80157-0.


Using immunoglobulin heavy chain transgenic mice, we show that B cell clones reaching the long-lived pool are heterogeneous: some are enriched in the CD21(high) compartment (mostly marginal zone [MZ]), others reside primarily in the follicles (FO). Altering the composition of the B cell receptor through N region additions decreases the rate of clonal production and the MZ enrichment. This process can be recapitulated by purified CD21(low) B cells and is due to a preferential clonal survival that requires a functional btk tyrosine kinase. We also show that generation of the MZ population is dependent on CD19. These findings suggest that the MZ B cell repertoire is positively selected and have functional implications for antigenic responses effected by B cells from this microenvironment.

Publication types

  • Research Support, U.S. Gov't, P.H.S.

MeSH terms

  • Agammaglobulinaemia Tyrosine Kinase
  • Animals
  • Antigens, CD19 / genetics
  • Antigens, CD19 / metabolism
  • Antigens, CD19 / physiology*
  • B-Lymphocytes / cytology*
  • B-Lymphocytes / immunology*
  • Bone Marrow Cells / cytology
  • Bone Marrow Cells / immunology
  • Cell Differentiation
  • Cells, Cultured
  • Clone Cells
  • Hematopoietic Stem Cells / cytology
  • Hematopoietic Stem Cells / immunology
  • Immunoglobulin Idiotypes
  • Immunophenotyping
  • Mice
  • Mice, Inbred BALB C
  • Mice, Inbred C57BL
  • Mice, Inbred CBA
  • Mice, Transgenic
  • Protein-Tyrosine Kinases / metabolism
  • Protein-Tyrosine Kinases / physiology*
  • Receptors, Antigen, B-Cell / metabolism
  • Receptors, Complement 3d / metabolism
  • Receptors, IgE / metabolism
  • Spleen / cytology


  • Antigens, CD19
  • Immunoglobulin Idiotypes
  • Receptors, Antigen, B-Cell
  • Receptors, Complement 3d
  • Receptors, IgE
  • Protein-Tyrosine Kinases
  • Agammaglobulinaemia Tyrosine Kinase