The expression of androgen receptors in human neck and limb muscles: effects of training and self-administration of androgenic-anabolic steroids

Histochem Cell Biol. 2000 Jan;113(1):25-9. doi: 10.1007/s004180050003.

Abstract

The purpose of this study was to investigate the immunohistochemical expression of androgen receptors (AR) in human vastus lateralis and trapezius muscles and to determine whether long-term strength training and self-administration of androgenic-anabolic steroids are accompanied by changes in AR content. Biopsy samples were taken from eight high-level power-lifters (P), nine high-level power-lifters who used anabolic steroids (PAS) and six untrained subjects (U). Myonuclei and AR were visualised in cross-sections stained with the monoclonal antibody against AR and 4',6-diamidino-2-phenylindole. The proportion of AR-containing myonuclei per fibre cross-section was higher in the trapezius than in the vastus lateralis (P<0.05). In the trapezius, the proportion of AR-containing myonuclei was higher in P compared to U and in PAS compared to both P and U (P<0. 05). On the contrary, in the vastus lateralis, there were no differences in AR content between the three groups. Myonuclear number in both muscles was higher in P compared to U and in PAS compared to both P and U (P<0.05). In conclusion, AR content differs greatly between human neck and limb muscles. Moreover, the regulation of AR-containing myonuclei following training and self-administration of androgenic-anabolic steroids is muscle dependent.

Publication types

  • Research Support, Non-U.S. Gov't

MeSH terms

  • Adult
  • Anabolic Agents / administration & dosage*
  • Cell Nucleus / metabolism
  • Humans
  • Leg
  • Male
  • Muscle, Skeletal / drug effects*
  • Muscle, Skeletal / metabolism*
  • Muscle, Skeletal / physiology
  • Neck Muscles / drug effects*
  • Neck Muscles / metabolism*
  • Neck Muscles / physiology
  • Receptors, Androgen / biosynthesis*
  • Self Medication
  • Testosterone Congeners / administration & dosage*
  • Weight Lifting / physiology*

Substances

  • Anabolic Agents
  • Receptors, Androgen
  • Testosterone Congeners