Atlantic salmon HNF-3/forkhead: cDNA sequence, evolution, expression, and functional analysis

DNA Cell Biol. 2000 Jan;19(1):59-68. doi: 10.1089/104454900314717.


We report the isolation and characterization of a cDNA encoding an HNF-3 family member (as HNF-3) from Atlantic salmon (Salmo salar L). The important functional domains of HNF-3 proteins that have been characterized previously are revealed by segments of high identity along the alignment of the asHNF-3 with winged helix/forkhead amino acid sequences isolated from other species. A comparison of asHNF-3 cDNA and genomic DNA indicated that there were no introns present in the asHNF-3 gene. Expression of asHNF-3 protein in adult salmon tissues was not exclusive to liver but was also present in the pancreas and intestine. An RT-PCR analysis performed on salmon development showed that asHNF3 expression is detectable before gastrulation at the mid blastula transition stage. Functional analysis of the asHNF-3 protein using a characterized HNF-3 consensus binding site demonstrated that the protein can recognize and bind to specific HNF-3 consensus sequences. We also report the identification of a novel HNF3 binding site in the promoter of the Atlantic salmon transferrin gene.

Publication types

  • Research Support, Non-U.S. Gov't

MeSH terms

  • Amino Acid Sequence
  • Animals
  • Base Sequence
  • Binding Sites
  • Conserved Sequence
  • DNA, Complementary / genetics
  • DNA, Complementary / isolation & purification
  • Evolution, Molecular
  • Forkhead Transcription Factors
  • Gene Expression Regulation, Developmental
  • Intestinal Mucosa / metabolism
  • Liver / metabolism
  • Molecular Sequence Data
  • Nuclear Proteins / genetics*
  • Nuclear Proteins / metabolism
  • Organ Specificity
  • Pancreas / metabolism
  • Phylogeny
  • Promoter Regions, Genetic
  • RNA, Messenger / analysis
  • Salmo salar / genetics*
  • Salmo salar / metabolism
  • Sequence Homology, Amino Acid
  • Transcription Factors / genetics*
  • Transcription Factors / metabolism
  • Transferrin / genetics


  • DNA, Complementary
  • Forkhead Transcription Factors
  • Nuclear Proteins
  • RNA, Messenger
  • Transcription Factors
  • Transferrin