Abstract
Escherichia coli FimH adhesin mediates binding to the bladder mucosa. In mice, a FimH vaccine protects against bacterial challenge. In this study, 4 monkeys were inoculated with 100 microgram of FimCH adhesin-chaperone complex mixed with MF59 adjuvant, and 4 monkeys were given adjuvant only intramuscularly. After 2 doses (day 0 and week 4), a booster at 48 weeks elicited a strong IgG antibody response to FimH in the vaccinated monkeys. All 8 monkeys were challenged with 1 mL of 108 E. coli cystitis isolate NU14. Three of the 4 vaccinated monkeys were protected from bacteruria and pyuria; all control monkeys were infected. These findings suggest that a vaccine based on the FimH adhesin of E. coli type 1 pili may have utility in preventing cystitis in humans.
Publication types
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Research Support, Non-U.S. Gov't
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Research Support, U.S. Gov't, P.H.S.
MeSH terms
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Adhesins, Bacterial / administration & dosage
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Adhesins, Bacterial / immunology*
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Adhesins, Escherichia coli*
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Animals
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Antibodies, Bacterial / blood*
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Bacterial Vaccines / administration & dosage
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Bacterial Vaccines / immunology*
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Escherichia coli / growth & development
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Escherichia coli / immunology*
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Escherichia coli / pathogenicity
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Escherichia coli Infections / microbiology
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Escherichia coli Infections / prevention & control*
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Feces / microbiology
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Fimbriae Proteins*
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Humans
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Macaca fascicularis
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Stomach / microbiology
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Urinary Bladder / microbiology
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Urinary Tract Infections / microbiology
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Urinary Tract Infections / prevention & control*
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Vaccination
Substances
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Adhesins, Bacterial
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Adhesins, Escherichia coli
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Antibodies, Bacterial
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Bacterial Vaccines
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fimH protein, E coli
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Fimbriae Proteins