Vaccination with FimH adhesin protects cynomolgus monkeys from colonization and infection by uropathogenic Escherichia coli

J Infect Dis. 2000 Feb;181(2):774-8. doi: 10.1086/315258.

Abstract

Escherichia coli FimH adhesin mediates binding to the bladder mucosa. In mice, a FimH vaccine protects against bacterial challenge. In this study, 4 monkeys were inoculated with 100 microgram of FimCH adhesin-chaperone complex mixed with MF59 adjuvant, and 4 monkeys were given adjuvant only intramuscularly. After 2 doses (day 0 and week 4), a booster at 48 weeks elicited a strong IgG antibody response to FimH in the vaccinated monkeys. All 8 monkeys were challenged with 1 mL of 108 E. coli cystitis isolate NU14. Three of the 4 vaccinated monkeys were protected from bacteruria and pyuria; all control monkeys were infected. These findings suggest that a vaccine based on the FimH adhesin of E. coli type 1 pili may have utility in preventing cystitis in humans.

Publication types

  • Research Support, Non-U.S. Gov't
  • Research Support, U.S. Gov't, P.H.S.

MeSH terms

  • Adhesins, Bacterial / administration & dosage
  • Adhesins, Bacterial / immunology*
  • Adhesins, Escherichia coli*
  • Animals
  • Antibodies, Bacterial / blood*
  • Bacterial Vaccines / administration & dosage
  • Bacterial Vaccines / immunology*
  • Escherichia coli / growth & development
  • Escherichia coli / immunology*
  • Escherichia coli / pathogenicity
  • Escherichia coli Infections / microbiology
  • Escherichia coli Infections / prevention & control*
  • Feces / microbiology
  • Fimbriae Proteins*
  • Humans
  • Macaca fascicularis
  • Stomach / microbiology
  • Urinary Bladder / microbiology
  • Urinary Tract Infections / microbiology
  • Urinary Tract Infections / prevention & control*
  • Vaccination

Substances

  • Adhesins, Bacterial
  • Adhesins, Escherichia coli
  • Antibodies, Bacterial
  • Bacterial Vaccines
  • fimH protein, E coli
  • Fimbriae Proteins