CXCR5-deficient mice develop functional germinal centers in the splenic T cell zone

Eur J Immunol. 2000 Feb;30(2):560-7. doi: 10.1002/1521-4141(200002)30:2<560::AID-IMMU560>3.0.CO;2-T.


The chemokine receptor CXCR5 is thought to be essential for the migration of B cells into the network of follicular dendritic cells in the spleen. However, as shown here, B cells and follicular dendritic cells do co-localize, albeit aberrantly, even in the absence of CXCR5. In mice lacking CXCR5 both cell types are found in a broad ring around the sinuses of the marginal zones. Upon immunization with the T cell-dependent antigen 2-phenyl-oxazolone, ectopic germinal centers develop in the periarteriolar lymphocyte sheath. A network of follicular dendritic cells forms in the vicinity of the central arteriole within which the antigen-activated B cells proliferate. The analysis of the expressed V gene repertoire revealed that during B cell proliferation, hypermutation is activated and V region genes accumulate somatic mutations. The pattern of somatic mutations suggests that affinity selection may occur. This analysis confirms that in CXCR5-deficient mice, the organization of splenic primary follicles is severely impaired. However, within the T cell zone a micro-environment is built up, which provides all requirements needed for the affinity maturation to take place.

Publication types

  • Research Support, Non-U.S. Gov't

MeSH terms

  • Animals
  • Cell Differentiation / immunology
  • Gene Expression Regulation / immunology
  • Germinal Center / cytology
  • Germinal Center / immunology*
  • Mice
  • Mice, Knockout
  • Mutation
  • Receptors, CXCR5
  • Receptors, Chemokine
  • Receptors, Cytokine / genetics
  • Receptors, Cytokine / immunology*
  • Spleen / cytology
  • Spleen / immunology*
  • T-Lymphocytes / immunology*


  • CXCR5 protein, mouse
  • Receptors, CXCR5
  • Receptors, Chemokine
  • Receptors, Cytokine