Transcripts and sequence elements suggest differential promoter usage within the ycf3-psaAB gene cluster on mustard (Sinapis alba L.) chloroplast DNA

Curr Genet. 2000 Jan;37(1):45-52. doi: 10.1007/s002940050007.

Abstract

The mustard chloroplast DNA region spanning the ycf3 gene and part of the psaAB operon was investigated. The ycf3 gene reveals two class-II introns that are removed during processing to give a mature 0.7-kb transcript, but no RNA editing seems to be involved. RNase protection and RT-PCR experiments suggest cotranscription of ycf3 with the downstream psaA gene, possibly from a NEP promoter upstream of ycf3, whereas distinct ycf3 and psaA transcripts are each initiated from PEP promoters. This situation is reminiscent of that for the trnK-psbA gene region. The implications for light-regulated versus light-independent expression of photosystem core-protein genes are discussed.

Publication types

  • Research Support, Non-U.S. Gov't

MeSH terms

  • Amino Acid Sequence
  • Base Sequence
  • DNA, Chloroplast / genetics*
  • Exons / genetics
  • Gene Expression Regulation, Plant
  • Genes, Plant / genetics*
  • Introns / genetics
  • Light-Harvesting Protein Complexes*
  • Membrane Proteins / genetics
  • Molecular Sequence Data
  • Mustard Plant / cytology
  • Mustard Plant / genetics*
  • Mustard Plant / growth & development
  • Operon / genetics
  • Photosynthetic Reaction Center Complex Proteins / chemistry
  • Photosynthetic Reaction Center Complex Proteins / genetics
  • Photosystem I Protein Complex*
  • Plant Proteins / genetics
  • Plants, Medicinal*
  • Promoter Regions, Genetic / genetics*
  • Promoter Regions, Genetic / physiology
  • RNA Splicing / genetics
  • RNA, Chloroplast / analysis
  • RNA, Chloroplast / genetics
  • RNA, Messenger / analysis
  • RNA, Messenger / genetics*
  • Sequence Alignment
  • Terminator Regions, Genetic / genetics
  • Terminator Regions, Genetic / physiology
  • Transcription, Genetic / genetics*

Substances

  • DNA, Chloroplast
  • Light-Harvesting Protein Complexes
  • Membrane Proteins
  • Photosynthetic Reaction Center Complex Proteins
  • Photosystem I Protein Complex
  • Plant Proteins
  • RNA, Chloroplast
  • RNA, Messenger
  • Ycf3 protein, Sinapis alba
  • photosystem I, psaB subunit

Associated data

  • GENBANK/AJ242660