Molecular characterisation of In51, a class 1 integron containing a novel aminoglycoside adenylyltransferase gene cassette, aadA6, in Pseudomonas aeruginosa

Biochim Biophys Acta. 1999 Dec 23;1489(2-3):445-51. doi: 10.1016/s0167-4781(99)00202-x.


Polymerase chain reaction-amplification and subsequent sequencing of the variable region of a novel integron, In51, from Pseudomonas aeruginosa revealed the presence of a novel aminoglycoside adenylyltransferase gene, aadA6, together with an open reading frame of unknown function, orfD. AADA6 enzyme has only 75% amino acid identity with AADA1 and is able to confer high level resistance to streptomycin and spectinomycin in Escherichia coli.

Publication types

  • Research Support, Non-U.S. Gov't

MeSH terms

  • Amino Acid Sequence
  • Bacterial Proteins / genetics*
  • Base Sequence
  • DNA, Bacterial
  • Molecular Sequence Data
  • Nucleotidyltransferases / genetics*
  • Pseudomonas aeruginosa / enzymology
  • Pseudomonas aeruginosa / genetics*
  • Sequence Homology, Amino Acid
  • Sequence Homology, Nucleic Acid


  • Bacterial Proteins
  • DNA, Bacterial
  • Nucleotidyltransferases
  • aadA6 protein, Pseudomonas aeruginosa

Associated data

  • GENBANK/AF140629