Structure, expression, and chromosome mapping of LATS2, a mammalian homologue of the Drosophila tumor suppressor gene lats/warts

Genomics. 2000 Jan 15;63(2):263-70. doi: 10.1006/geno.1999.6065.

Abstract

We have cloned and characterized LATS2, a novel mammalian homologue of the Drosophila tumor suppressor gene lats/warts. Northern blot analysis showed ubiquitous expression of mouse LATS2 (MmLATS2) mRNA, whereas expression of human LATS2 (HsLATS2) mRNA was enhanced in skeletal muscle and heart. Immunoblotting analysis of fractionated cell lysates showed HsLats2 to be a nuclear protein. We mapped the MmLATS2 gene to mouse chromosome 14 by interspecific backcross analysis. We also mapped the HsLATS2 gene (by fluorescence in situ hybridization) to the 13q11-q12 region, in which a loss of heterozygosity has been frequently observed in many primary cancers and to which the tumor suppressor genes RB and BRCA2 have also been mapped.

Publication types

  • Comparative Study
  • Research Support, Non-U.S. Gov't
  • Research Support, U.S. Gov't, P.H.S.

MeSH terms

  • Amino Acid Sequence
  • Animals
  • Chromosome Mapping
  • Chromosomes, Human, Pair 13 / genetics
  • DNA, Complementary / genetics
  • DNA, Complementary / isolation & purification
  • Drosophila / genetics
  • Drosophila Proteins*
  • Gene Expression
  • Genes, Insect
  • Genes, Tumor Suppressor*
  • Humans
  • In Situ Hybridization, Fluorescence
  • Mice
  • Molecular Sequence Data
  • Phylogeny
  • Protein Kinases / genetics*
  • Protein-Serine-Threonine Kinases / genetics*
  • RNA, Messenger / genetics
  • RNA, Messenger / metabolism
  • Sequence Homology, Amino Acid
  • Tumor Suppressor Proteins*

Substances

  • DNA, Complementary
  • Drosophila Proteins
  • RNA, Messenger
  • Tumor Suppressor Proteins
  • Protein Kinases
  • LATS1 protein, human
  • Lats1 protein, mouse
  • wts protein, Drosophila
  • LATS2 protein, human
  • Protein-Serine-Threonine Kinases

Associated data

  • GENBANK/AB023958
  • GENBANK/AB028019