Coordinate recruitment of E-cadherin and ALCAM to cell-cell contacts by alpha-catenin

Biochem Biophys Res Commun. 2000 Jan 27;267(3):870-4. doi: 10.1006/bbrc.1999.2040.


Here we report on the role of alpha-catenin in the cellular localization of activated leukocyte cell adhesion molecule, ALCAM, and cadherin-mediated cell adhesion in human prostate cancer cells. Cell lines that have a functional E-cadherin-mediated cell adhesion (DU-145 and LNCaP) show ALCAM staining at cell-cell contacts. In contrast, in cell lines that lack alpha-catenin expression (ALVA-31, PC-3, and PPC-1), E-cadherin-mediated adhesion is disturbed and ALCAM staining is cytoplasmic. A role of alpha-catenin in the recruitment of E-cadherin and ALCAM to cell-cell contacts was established by transfection of an alpha-N-catenin construct into cell lines ALVA-31 and PC-3. This resulted not only in the correct assembly of E-cadherin/alpha-catenin complexes at the cell membrane but also in localization of ALCAM to cell-cell contacts, indicating that indeed alpha-catenin affects ALCAM localization.

MeSH terms

  • Activated-Leukocyte Cell Adhesion Molecule / analysis
  • Activated-Leukocyte Cell Adhesion Molecule / biosynthesis
  • Activated-Leukocyte Cell Adhesion Molecule / physiology*
  • Cadherins / analysis
  • Cadherins / physiology*
  • Cell Adhesion / physiology*
  • Cytoskeletal Proteins / analysis
  • Cytoskeletal Proteins / biosynthesis
  • Cytoskeletal Proteins / physiology*
  • Humans
  • Immunohistochemistry
  • Male
  • Prostatic Neoplasms
  • Recombinant Proteins / analysis
  • Transfection
  • Tumor Cells, Cultured
  • alpha Catenin


  • Activated-Leukocyte Cell Adhesion Molecule
  • CTNNA1 protein, human
  • Cadherins
  • Cytoskeletal Proteins
  • Recombinant Proteins
  • alpha Catenin